Puromycin invitrogen
WebInvitrogen™ Custom DNA Oligos Tool; Invitrogen™ Custom RNAi Tool; Assay Tools. ... Formula: C22H29N7O5 • 2HCl, Purity: ≥ 98% (HPLC), Molecular weight: 544.3, Endotoxin … WebApr 14, 2024 · Invitrogen: Cat#L3000001: Phusion High-Fidelity DNA Polymerase with dNTPs: ... The vector contains an anti-puromycin gene as selectable marker and the EGFP …
Puromycin invitrogen
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WebThe TrkB-expressing pBabe-puro (pBP) vector was a kind gift fromDr.DanielS.Peeper(DivisionofMolecularGenetics,the Netherlands Cancer Institute, Amsterdam, the Netherlands); the pLKO.1 expression vector, packaging plasmid psPAX2, and envelope plasmid pMD2.G for producing viral particles … WebApr 13, 2024 · After infection, the cells were cultured with 2 μg/mL puromycin to select for stably transduced cells. For A375 rescue ... (Invitrogen) and either a human HS6ST2-HA expression plasmid (Applied Biological Materials Inc.), a human GPC4-HA expression plasmid (Sino Biological), or an siRNA targeting human SULF1 (SASI_Hs02_00330796 ...
Webpuromycin resistance gene RI random integration RMCE recombinase‐mediated cassette exchange UCOE ubiquitous chromatin ... Invitrogen). After 2–3 days of transfection, … WebPuromycin is weakly active against Gram-negative microorganisms. Resistance to puromycin is conferred by the pac gene, which encodes a Puromycin N-acetyl-tranferase …
WebApr 10, 2024 · Following 3 washes in PBS, the fluorescently conjugated secondary antibodies were applied (Invitrogen, 1:1000) and co-cultured with the cells for 30 min at RT. Slides were mounted prolong gold anti-fade reagent with DAPI (Invitrogen, USA) after being washed with PBS for 3 times and imaged on an LSM880 confocal microscope (ZEISS, …
WebMedium containing 1 μg/mL puromycin was used to select the stable cells. Transient-Transfected Cell Construction. The miR-135b, miR-186-5p and miR-218-5p mimics …
WebProvided herein are compositions, systems, and methods for controlling gene expression and epigenetic memory utilizing synthetic fusion proteins comprising a DNA binding protein or domain or fragment thereof and a nanobody configured to bind a transcriptional regulator (e.g., a chromatin regulator). glider schools seattleWebPuromycin: Sigma: P8833: Ethylenediamine tetraacetic acid (EDTA) Sigma: 139-33-3: Bovine Serum Albumin (BSA) Sigma: A2153-100G: Matrigel: ... Sigma) to avoid cell adhesion. They were cultivated in DMEM/F12 1:1 (Invitrogen), N2 and B27 supplements (Invitrogen), 2 mM glutamine (Invitrogen), 0.6% glucose (Sigma), 20 μg/ml bovine insulin (Sigma ... gliders and reclinersWebDec 30, 2024 · Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose … glider school dayville ctWebApr 12, 2024 · Puromycin (0.5 μg/ml) was added at 48 hours after infection to select infected cells. After 7 days, puromycin was removed, and the cells were allowed to recover in regular growth ... For RNA extraction, 1 ml of TRIzol (Invitrogen, 15596-018) was added to homogenized brain tissue or cells following the standard TRIzol protocol ... gliders and rocking chair reviews blogWebPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, [1] that causes premature chain termination during translation taking place in … bodystreet trainingWebVirus by retroviral-mediated genes transfer are either pBABE-puro-H-RasG12V otherwise empty vector were performed as previously described (Bischof to al., 2006) Growth protocol: Culturing of human diploid WI38 fibroblasts ... Invitrogen). This became followed by back stripe cDNA synthesis using Polymerase I and RNase H. glider school near bostonWebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It is a very common antibiotic routinely used by scientists in biomedical research to select cells … glider school cost