WebAfter incorporating a pulse of a labelled nucleotide , such as 5-bromouridine 5′-triphosphate (BrUTP) or biotin-CTP, into permeabilized mammalian cells, Cook and colleaguesnoted … WebNov 1, 2024 · In this study, we designed a biotinylated CTP affinity probe which harbors three components: CTP, a binding moiety, which specifically recognizes the active sites of CTP-binding proteins; biotin, an enrichment moiety, which facilitates the enrichment of target proteins; γ-aminobutyric acid, a linker, which connects the enrichment moiety to …

Invitrogen™ Biotin-14-CTP - Fisher Sci

WebBiotin-11-CTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace CTP in the in vitro transcription reaction for RNA labeling. Double-stranded DNA ( i.e., linearized plasmid or cDNA) containing a T7, SP6, or T3 promoter serves as a template for in vitro transcription with the corresponding RNA polymerase, using ATP, GTP, CTP, UTP ... WebTech Note. Simple, rapid streptavidin-based enrichment using Capturem technology. 5- to 15-minute enrichment protocol of biotinylated compounds, including antibodies, proteins, and nucleic acids. Available in 96-well plates for high-throughput applications, compatible with centrifugation or vacuum and positive pressure automated systems. heather lindsey purpose

Biotin-11-dCTP, Biotin-labeled Cytidines - Jena …

WebBiotin-14-CTP is a CTP analog that contains biotin attached at the N4-position via a 14-atom linker. It is provided as a 10 mM solution in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template) using T7 RNA WebBiotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA … heather lindsey tmz