WebJan 27, 2010 · The luminescent ADP-Glo™ assay has been developed as an alternative to radiometric format for assaying protein kinase targets. This assay allows the measurement of the ADP product formed during the kinase reaction. Therefore, the luminescent ADP-Glo assay is similar to the radiometric format in that it measures the direct product of the ... WebDec 1, 2009 · ADP-Glo is a novel bioluminescent, homogeneous assay for monitoring ADP producing biochemical reactions and thus it is an ideal assay for detecting enzyme activity using a wide variety of...
Phosphatase-Coupled Universal Kinase Assay and Kinetics for …
WebWe recently evaluated the ADP-Glo assay from Promega. This is a homogeneous, signal increase assay that measures ADP production from a kinase reaction by coupled enzymes that first convert ADP to ATP and subsequently quantifies ATP using luciferase in the presence of luciferin. Since the unused ATP in the reaction is depleted prior to ADP to ... WebAug 11, 2011 · When ADP and ATP concentrations were significantly below the K m values (<0.15 mM), CD39L2 hydrolysis of both substrates exhibited the kinetics of a first-order … pilot holes wood screws
Federation Redirector - ADP
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